Rootchip微流控系统

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August undefined, 2024

WebJul 7, 2012 · @article{osti_1628632, title = {Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip}, author = {Grossmann, Guido and Meier, Matthias and Cartwright, Heather N. and Sosso, Davide and Quake, Stephen R. and Ehrhardt, David W. and Frommer, Wolf B.}, abstractNote = {The root … Web定义. 微流控（Microfluidics）指的是使用微管道（尺寸为数十到数百微米）处理或操纵微小流体（体积为纳升到阿升）的系统所涉及的科学和技术，是一门涉及化学、流体物理、微 …

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth …

WebMar 27, 2024 · The RootChip design uses a two-layer design with a built-in valve system to divide a single fluid input into the different channels. Although this design has several … WebMar 7, 2012 · The roots continue to grow into the RootChip’s observation chambers which are filled with liquid medium. The microfluidic channel that leads from the inserted tip transitions from vertical to horizontal and the root typically aligns with the direction of flow. The entire RootChip is encased in a chip carrier that includes water reservoirs to ... hawaiian luau dinner party games

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth …

WebDec 13, 2024 · One sentence summary: Bi-directional-dual-flow-RootChip to track calcium signatures in Arabidopsis primary roots responding to osmotic stress.Plant growth and survival is fundamentally linked with the ability to detect and respond to abiotic and biotic factors. Cytosolic free calcium (Ca2+) is a key messenger in signal transduction pathways … WebDec 23, 2013 · The RootChip enables growth of seedlings in a highly controlled microenvironment and direct microscopic observation of growing roots. The chip perfusion system provides growth medium during seedling development and features micromechanical valves that allow pulsed treatments of roots. The RootChip allows the … WebJan 10, 2024 · One sentence summary: Bi-directional-dual-flow-RootChip to track calcium signatures in Arabidopsis primary roots responding to osmotic stress. Plant growth and survival is fundamentally linked with the ability to detect and respond to abiotic and biotic factors. Cytosolic free calcium (Ca 2+) is a key messenger in signal transduction … hawaiian luau disney

RootChip: An Integrated Microfluidic Chip for Plant Science

Microfluidic systems for plant root imaging - ScienceDirect

Webside to the bottom of the RootChip base. 20)Homogeneously press the tape against the RootChip base using a foil squeegee. 21)Remove the second part of the double-sided adhesive tape and align it onto the glass substrate. 22)Homogeneously press the RootChip base against the glass substrate for approximately 1 minute. For the custom humidity … Web最后，研究人员使用简化版的RootChip微流控系统结合可以快速改变磷酸盐溶液能力的活细胞成像技术，并采用了旋转圆盘显微镜，持续的观察到去Pi溶液导致SPX1启动子的破 … hawaiian luau dinner ideasWebThe RootChip is fabricated from polydimethylsiloxane (PDMS) and uses integrated micromechanical push-up valves to guide ﬂuid ﬂow (Unger et al., 2000). The ﬂow layer … hawaiian luau dinner and show september 10

"WebDec 20, 2011 · The RootChip has separate chambers for individual regulation of the microenvironment of multiple roots from multiple seedlings in parallel. We demonstrate the utility of The RootChip by monitoring time-resolved growth and cytosolic sugar levels at subcellular resolution in plants by a genetically encoded fluorescence sensor for glucose … " - Rootchip微流控系统

Rootchip微流控系统

WebDec 20, 2011 · The RootChip can be modified for use with roots from other plant species by adapting the chamber geometry and facilitates the systematic analysis of root growth and … WebJul 7, 2012 · The RootChip, a microfluidic chip platform, allows root growth in a controlled environment on the microscope Grossmann et al., 2012). Combined imaging using FRET sensors with the RootChip can ...

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WebThe RootChip can be modified for use with roots from other plant species by adapting the chamber geometry and facilitates the systematic analysis of root growth and metabolism … WebNov 10, 2024 · Using the dual-flow-RootChip, we revealed cell-autonomous adaption of root hair development under asymmetric phosphate (Pi) perfusion, with unexpected repression in root hair growth on the side exposed to low Pi and rapid tip-growth upregulation when Pi concentrations increased. The asymmetric root environment further resulted in an …

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WebJan 1, 2024 · The RootChip-8S is designed to provide a protected growth environment with optimal access for high-resolution imaging, enabling control over the chemical conditions through perfusion. While any damage caused by specimen mounting is avoided, growing roots inside a narrow chamber is certainly not free from potential pitfalls. WebDec 28, 2011 · The RootChip also revealed the long-suspected fact that galactose, a sugar highly similar to glucose, is toxic to roots and inhibits their growth and function. The RootChip is a generic tool and can be altered to test any aspect of root physiology that can be analyzed visually. It can easily be modified to study more than 30 seedlings at the ...

WebDownload scientific diagram Establishing asymmetric root microenvironments in the dual-flow-RootChip. (a) Uptake of fluorescein upon asymmetric perfusion of an Arabidopsis root over time (time ...

WebMethods Dual-ﬂow-RootChip reveals local adaptations of roots towards environmental asymmetry at the physiological and genetic levels Claire E. Stanley1,2*, Jagriti Shrivastava3,4*, Rik Brugman3, Elisa Heinzelmann3, Dirk van Swaay1 and Guido Grossmann3,5 1Institute for Chemical and Bioengineering, ETH Z€urich, Vladimir-Prelog … hawaiian luau dress ideasWebThe RootChip can be modified for use with roots from other plant species by adapting the chamber geometry and facilitates the systematic analysis of root growth and metabolism … hawaiian luau drinks alcoholicWebDec 28, 2024 · 执行过程：. 下载源码；. 配置、编译、安装交叉工具链；. 配置、编译、安装选择的包；. 按选择的格式生成根文件系统；. Buildroot 输出结果保存在 output 目录，具 … hawaiian luau dinner recipesWebDec 1, 2011 · The RootChip can be modified for use with roots from other plant species by adapting the chamber geometry and facilitates the systematic analysis of root growth and … hawaiian luau dressesWebSep 20, 2024 · This protocol provides a detailed description of how to fabricate and use the dual-flow-RootChip (dfRootChip), a novel microfluidic platform for investigating root nutrition, root-microbe interactions and signaling and development in controlled asymmetric conditions. The dfRootChip was developed pri … hawaiian luau disney worldWebDownload scientific diagram On-Chip Quantification of Sugar Levels Using a FRET Nano- sensor. from publication: The RootChip: An Integrated Microfluidic Chip for Plant Science Studying ... hawaiian luau dresses for kidsWebMar 21, 2024 · RMSL201-1301. 专业DSP深度计算，精度高，环境兼容性高，灵活性高。. 提供刷脸支付全栈方案，DSP到AI芯片，深度算法到光学设计，全方位支持。. 针对人像 … hawaiian luau dinner show orlando