Pcold 1
SpletpCold™ベクターシリーズ. 大腸菌のコールドショック発現系. 培養液を15℃に冷却するだけで効率よく発現を誘導. 従来の大腸菌発現系に比べ、生産効率と可溶性発現が向上. … Splet冷休克表达载体，pCold I-IV，是利用冷休克基因CSPA启动子设计高效率蛋白质表达载体。 CSPA的启动子的下游处，插入了lac操纵子，以便严格控制表达。 此外，5'非翻译区（5'UTR），翻译增强元件（TEE），His标签序列，Xa因子切割位点和多克隆位点（MCS）位于在CSPA启动子的下游的。 由于该产品采用了大肠杆菌的启动子，大多数大 …
Pcold 1
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SpletpCold-R 20-mer / 50% GC 1 binding site 369 .. 388 = 20 annealed bases T m = 56°C TEE 256 .. 270 = 15 bp 5 amino acids = 627.8 Da Product: translation enhancing element for E. coli … http://www.procoldinc.com/
SpletpCold-R 20-mer / 50% GC 1 binding site. 336 .. 355 = 20 annealed bases T m = 56°C: lacI 3114 .. 4196 = 1083 bp 360 amino acids = 38.6 kDa. Product: lac repressor. The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). ... SpletpCold III Vector for cold shock-induced expression in E. coli of a protein with a translation enhancing element. Sequence Author: TaKaRa Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Basic Cloning Vectors More Plasmid Sets Home Plasmids Basic Cloning VectorspCold III Show Static …
http://www.biofeng.com/zaiti/dachang/pColdS-SUMO.html SpletpCOLADuet-1 Sequence and Map Bacterial vector with a ColA origin for the co-expression of two genes. Bacterial vector with a ColA origin for the co-expression of two genes. …
Splet02. sep. 2024 · pCold DNA I ～IV 是应用冷休克基因之一的cspA 基因启动子而设计并 可以有效表达蛋白质的冷休克表达载体。 在cspA 启动子下游插入了 lac operator ，可以严紧调 …
SpletpColdベクターはコールドショック遺伝子の一つであるcspA遺伝子のプロモー ターを利用したコールドショック発現ベクターで、cspAプロモーターの下流に5'非翻訳領域（5' … fun activities for christmas in officeSpletOur pCold TF DNA vector is a fusion cold shock expression vector that expresses trigger factor (TF) chaperone as a soluble fusion tag. Trigger factor is a prokaryotic ribosome-associated chaperone protein of 48 kDa which facilitates co-translational folding of nascent polypeptides, reducing protein misfolding and production of insoluble protein. girasoles wallpaper pcSpletThere are four types of pCold vectors, the location of their following parts is slightly different, TEE, His tag sequence and Factor XA enzyme site. PColdI plasmid is designed … girasole theatreSpletpros相关信息，ProSight Global PROSPROS_狒狒了，微博原创视频博主。PROS_狒狒了的微博主页、个人资料、相册,浙江理工大学。新浪微博，随时随地分享身边的新鲜事儿。 fun activities for children in londonSpletpCold TF载体由CSPA启动子及其下游的序列组成，其下游序列主要包括包括5'非翻译区（5'非编码区），翻译增强元件（TEE），His标签序列，多克隆位点（MCS）。 Lac操纵子插入在CSPA启动子下游，以确保严格的表达调控。 此外，HRV 3C蛋白酶，凝血酶，和Factor Xa识别位点位于“Trigger Factor”和多克隆位点（MCS）之间，以便表达的融合蛋白 … girasole pittsburgh menuSpletpCold-R 20-mer / 50% GC 1 binding site. 336 .. 355 = 20 annealed bases T m = 56°C: lacI 3114 .. 4196 = 1083 bp 360 amino acids = 38.6 kDa. Product: lac repressor. The lac … fun activities for club meetingsSpletSeason 1 - Cold Podcast Season 1: Susan Powell Case Files Prelude Susan Powell disappeared from her home in December of 2009. Her body has never been found. The man suspected of killing her was never arrested or charged. This begins the complete story of the search for Susan. Read More Ep 1: To All the Girls I’ve Loved Before Boy meets girl. girasoles sunflower farm