Creb western blot
WebSep 29, 2024 · CREB-2/ATF-4 Antibody (B-3) is recommended for detection of ATF-4 of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA Anti-CREB-2/ATF-4 …
Creb western blot
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WebCREB was first described in 1987 as a cAMP-responsive transcription factor regulating the somatostatin gene. Genes whose transcription is regulated by CREB include: c-fos, the … WebAug 19, 2011 · Decrease in CREB protein levels of Alzheimer's mouse hippocampal neurons. Western blot analysis of CREB and correction of band intensities with β actin levels showed 40% decrease (p < 0.01) in Tg2576 mouse hippocampus as compared to control samples (Figure 2A and 2B).The levels of synaptophysin, a neuronal protein, did …
Web方法应用Western blotting法检测APP和cAMP效应元件结合蛋白(cAMP responsive element-binding protein,CREB)的表达。 用Matinspector软件分析APP启动子区CRE顺式反应元件；构建不同长度APP启动子区荧光素酶表达质粒；用双荧光素酶报告基因系统检测细胞中荧光 … WebWestern blot analysis of CREB was performed by loading 30 µg of K562 and Jurkat cell lysates using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # …
WebCREB synonyms, CREB pronunciation, CREB translation, English dictionary definition of CREB. n cyclic amp-response element binding protein; a protein involved in the long … WebDec 1, 2024 · To investigate the role of CREB in Sb-induced neuronal apoptosis, we evaluated CREB phosphorylation levels by immunoblotting after treating PC12 cells with 0, 25, 50, and 75 μM Sb for 24 h. Sb treatment increased the levels of CREB phosphorylation in a dose-dependent manner; the total CREB levels were not affected by Sb treatment ( …
WebJul 3, 2024 · The P38/CREB/MMP13 axis is active in osteoarthritic chondrocytes and may cause the degeneration of cartilage. Effective new therapy directed against this pathway could be developed. ... Phosphorylated (p)-P38, p-CREB, and MMP13 were evaluated by Western blot analysis. Anisomycin was used to activate P38, and p-P38, p-CREB, and …
WebFeb 1, 2008 · CREB knockdown was 65% by Western blot analysis after 21 days in methylcellulose (Figure 2Bi). We observed a 3-fold decrease in CFU-GM colony formation in methylcellulose with CREB shRNA–transduced cells compared with control cells (P < .05; Figure 2Bii). Cytospin assays confirmed that CFU-GM were in the form of immature … the nubaWebCREB Antibodies Antibodies that detect CREB can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Flow Cytometry. These antibodies target CREB in Human, Mouse, Rat, Zebrafish and Non-human primate samples. michigan medicine office of researchWebP38/CREB/MMP13 is active in ANS-induced human and mouse chondrocytes. Primary chondrocytes in the CON group were isolated from control cartilage and normal mice. … michigan medicine oheiWebSep 15, 2014 · p16 Ink4a downregulation increases the phosphorylation of CREB. Western blots show higher increase of p-CREB in primary hepatocytes isolated from p16 −/− compared with p16 +/+ mice and treated with glucagon for 1 h (A) and in p16 Ink4a-silenced compared with p16 Ink4a-expressing AML12 cells treated with 10 μmol/L forskolin (FSK) … michigan medicine nursing positionsWebP38/CREB/MMP13 is active in ANS-induced human and mouse chondrocytes. Primary chondrocytes in the CON group were isolated from control cartilage and normal mice. ANS was used to activate the P38 signal in the chondrocytes. Then p-P38, p-CREB, and MMP13 were evaluated by immunofluorescence and Western blot analysis. the nubble bustins islandWebMar 7, 2005 · Using CREB:DNA complexes obtained from HEK293T cells by ChIP to screen the Hu19K array ( P ≤ 0.001, binding ratio ≥2), we found ≈3,000 CREB-positive promoters, accounting for nearly 20% of protein coding genes (2,811/16,361 or 17%; see Table 6, which is published as supporting information on the PNAS web site, and … the nuba survivalWebCREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular … michigan medicine obgyn residency